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KMID : 0350519930460020713
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Journal of Catholic Medical College
1993 Volume.46 No. 2 p.713 ~ p.727
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Correlation of P-Glycoprotein Expression, DNA Ploidy and Pathologic Parameters in Human Breast Cancer
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Abstract
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DNA analysis and immunohistochemical staining of [-glycoprotein were performed in paraffin embedded tumor samples from 45 breast carcinomas(6 invasive ductal carcinomas, well differentiated ; 20 invasive ductal carcinomas, moderately
differentiated
; 6
invasive ductal carcinomas, poorly differentiated ; 4 invasive ductal carcinomas with prominent ductal compont ; 2 comedocarcinomas ; 2 medullary carcinomas ; 5 invasive ductal carcinomas, scirrhous type).
The 38 benign breast tumor samples and 3 marrow tissue sections from acute monocytic leukemia were used as control group in DNA analysis and in immunohistochemical staining for P-glycop[rotein, respectively.
DNA proliferative fractions were calculated by bitmap gating using flow cytometry in S, G2, M phases. Aneuploidy was determined from the DNA histogram using linear S-phase method. and pglycoprotein expressed in certain breast carcinoma was
studied
by
immunoperoxidase staining. Possible relationships between these two indices, DNA proliferative variables and P-glycoprotein expressron rate, and a number of pathological parameters were investigated in the breast carcinomas The pathological
parameters
assessed were cell types, lymph node metastasis, tumor size, nuclear grade, structural grade, mitotic index and growth pattern.
@ES The result were as follows ;
@EN 1. There was a negative correlation between DNA content(G0G1) in aneuploid tumors and lymphnode metastasis(r=-0.432, P=0.028).
2. P-glycoprotein expression rate in 45 breast carcinomas were 22 positive(48.9%) and 23 negative(51.1%). Comparing P-glycoprotein with pathologic parameters, there was a significant relationship with P-glycoprotein expression rate and in tumor
sizes,
structural abnormalities, (Bartholomew test, P<0.05) and nuclear grades(X2=09.32, P=0.009).
3. The P-glycoprotein expression rate of 26 DNA aneuploid tumors was 65.4%(17cases). There was a significant relationship with P-glycoprotein expression rate, and in various nuclear grades and structural abnomalities(Bartholomew test, P<0.05).
4. Comparing P-glycoprotein expression rate with DNA ploidy, 5 cases were P-glycoprotein positive in 19 DNA diploid tumors, and 17 cases were positive in 26 DNA aneuploid tumors. There was a significant relationship with P-glycoprotein and DNA
ploidy(X2=6.71, P=0.010).
5. Among 45 breast carcinomas, 21 cases, lymph node metastasized, were composed of 5 DNA diploidy and 16 DNA aneuploidy. There was a significant relationship with lymph node metastasis and DNA ploidy(X2=5.47, P=0.019).
In conclusion, P-glycoprotein expression rate was high in association with increased nuclear grade, structural grade and tumor size in breast carcinoma. There was higher lymph node metastasis and increased P-glycoprotein expression rate in
aneuploid
tumors. Aneuploid tumors have poor prognosis and also suggested their strong relationship with drug resistance.
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